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Complete information on authors, methodology and conflicts of interest is available in the published paper

One milliliter of a PBS-based reaction mixture containing propidium iodide (20 g/mL), RNase (0.2 g/mL), and 0.1% Triton X-100 (Sigma) was added to the cell samples at a density of 1 10 6 cells/mL and incubated in the dark for 40 min before a DNA content analysis was conducted through flow cytometry

Key nutrients to prioritize: Protein to protect muscle mass Fiber (2530 g/day for women) to support fullness and prevent constipation Hydration Micronutrients such as calcium, iron, and vitamin D Hormonal shifts can further influence digestion and metabolism, particularly in women navigating overlapping conditions such as PCOS and perimenopause, explored in The PCOSPerimenopause Connection

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