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Using one needle for both caused itch problem
From the ASV table (Supplementary Table S1), sums of ASVs within each respective phylum or genus for each sample were calculated to establish the abundance of each phylum or genus per sample
The purpose of this treatment is to eliminate matrix constituents that could interfere with the detection process or damage analytical equipment, and to enrich the analyte in the sample so that it is readily available for detection [3], [2]
This depletion can lead to cramps, insomnia etc
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