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X-ray diffraction data were collected at the PXIII beamline of the Swiss Light Source, Paul Scherrer Institute (Villigen, Switzerland)
p53 TAD (13-61) was subcloned into the pET His 6 glutathione-S-transferase (GST) TEV LIC cloning vector (2G-T, obtained from Scotta Gradia, Addgene plasmid #29707) and transformed into BL21 (DE3) pLysS cells
The long read lengths characteristic of third-generation sequencing technologies enable researchers to resolve complex genomic structures more effectively, particularly excelling in animal genome assembly
Given the multifactorial nature of glaucoma-associated ocular surface disease (G-OSD)which involves chronic inflammation, oxidative stress, and tear film instabilitydeveloping novel therapies that target these intersecting pathways is of considerable clinical relevance
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