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Therefore, pale skin was desired, and the use of bleaching creams or white facial makeup, sometimes with toxic ingredients, was not uncommon

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LC-MS/MS conditions The analyte molecules were eluted on Zorbax XDB-C18 column (3.5 m, 2.1x20 mm) with the mobile phase composed of water/0.1% formic acid and methanol/0.1% formic acid in the ratio of 70:30 V/V with the flow rate of 0.2 ml/min

Peptides were eluted over a 120-min gradient with buffer A as 100% water + 0.1% formic acid and buffer B as water + 0.1% formic acid and acetonitrile with the following steps: 05% B for 1 min, 525% for 68 min, 2540% for 20 min, 4060% for 15 min, 6095% for 1 min and 95% for 15 min