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doi: 10.3109/00365599.2011.585622 233

Western blot analysis and immunoprecipitation Total cells lysates for immunoblotting or immunoprecipitation were lysed with buffer (0.1%Triton-100, 50 mM Tris-Cl, pH 7.5, 0.1% NP-40, 150 mM NaCl, 0.1 M EDTA) supplemented with a cocktail of phosphate and proteinase inhibitors for 30 min at 4 C

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For example, rodent studies reveal that labeled semaglutide accumulates predominantly in brainstem, septal nucleus, and hypothalamus, areas with more permissive BBB permeability (Gabery et al., 2020)