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Think about what happens when you take a vitamin pill
Transfection of overexpressing or siRNA plasmids were carried out in 6070% confluent cells by using Lipofectamine 3000 reagent according to the manufacturers protocol
In the present study, it is noted that the expression of ferritin (FTH1), a protein functioning in maintaining iron homeostasis, was significantly decreased at the protein level, indicating that riboflavin deficiency can impact significantly on iron absorption and utilization 48,49
Since this process also appears to be regulated via a negative feedback mechanism, the ancillary impacts typically associated with excessive growth hormone production appear to be avoided
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