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Figure 4 Figure 5 3.5 Cystine increases intracellular ferrous ion concentrations and elevates ROS levels following gentamicin treatment Despite their specific drugtarget interactions, bactericidal antibiotics are known to stimulate the production of highly deleterious hydroxyl radicals in bacteria, a process that is facilitated through the Fenton reaction involving intracellular ferrous iron ( Therefore, we measured ROS levels following gentamicin treatment in the presence or absence of cystine via a DCFH-DA fluorescent probe