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Cells were seeded at a density of 5000 cells per well in 200 l fresh culture medium (DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution) into 96-well flat-bottomed microtitre plates (Costar, Corning, USA) and incubated at 37 C in humidified 5% CO 2 using AutoFlow NU-5510 Direct Heat CO 2 Incubator (NuAire, USA) for 24 hours before treatment
Regulatory and compounding status All three compounds IGF-1 LR3, DES(1-3) IGF-1, and PEG-MGF share the same regulatory status in the United States: none have FDA-approved pharmaceutical formulations
Future studies are warranted to explore more targeted GSH supplementation and its effect on treatment efficacy
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