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The four-pathway model therefore targets appetite (GLP-1 and amylin through separate neuronal circuits), fat metabolism (GIP and glucagon), energy expenditure (glucagon primarily), and glucose homeostasis (GLP-1, GIP, and amylin all contribute through different mechanisms)
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Amblard, M., Fehrentz, J
The protein was solubilised using n-dodecyl -D-maltoside (DDM) and cholesteryl hemi-succinate (CHS), which was then exchanged for glyco-diosgenin (GDN) during purification
