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Western immunoblot analyses For the preparation of total liver homogenates, frozen liver pieces were homogenized in RIPA buffer (150 mM NaCl, 1% TritonX-100, 0.25% sodium deoxycholate, 0.1% SDS, 50 mM Tris, 1 mM EDTA, 1 mM PMSF, protease inhibitor cocktail, pH 7.4) on ice with a tissue tearor (BioSpec Products, Bartlesville)
(21(4)):479-493 doi:10.1007/s11154-020-09568-3
Although the therapeutic dose of liraglutide appeared to enhance bone material properties, the most significant improvements in BMD and microarchitecture were noted at doses much higher than those used for weight loss in PwO
10.1089/ars.2016.6866 82 YuF.YuC.LiF.ZuoY.WangY.YaoL.et al (2021)
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