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Curr Develop Nutr 2024;8(12):104500
After denaturing in a boiling water bath, the proteins were separated by SDSpolyacrylamide gel electrophoresis (SDSPAGE), transferred to a PVDF membrane, blocked with 5% milk in TBST for 30 min at room temperature, and then incubated with primary antibody at 4 C overnight with shaking
Its effects should be considered supportive, not therapeutic, and outcomes may vary between individuals
For an avascular structure like a disc, even modest improvements in surrounding blood flow could meaningfully increase nutrient delivery
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