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Dissecting redox biology using fluorescent protein sensors
55 participants had bilateral thalamic data, while 21 participants had only left thalamic data due to time constraints or positioning issues with the right thalamic voxel
After 20 min of centrifugation at 1,520 rcf, 2 mL of supernatant was mixed with 2 mL of acid ninhydrin reagent and incubated at 100C in a water bath (OLS Aqua Pro Shaking Water Bath, Grant Instruments, Cambridge, UK) for 1 h
The data obtained from the run were analyzed and normalized using the qBASE software, employing the 2 Ct method as described by Livak and Schmittgen (2001) and Hellemans et al
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