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85 MTX requires regular monitoring to optimize dosing and assess its immunosuppressive and hepatotoxic effects through frequent blood tests (monthly, initially)
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The recommended daily intake of biotin is 30100 mcg, though higher doses (up to 10,000 mcg) are commonly used in hair growth supplements with no reported toxicity
Analysis was performed using an EASY-nLC 1000 system (Thermo Fisher Scientific) coupled to a QExactive mass spectrometer (Thermo Fisher Scientific) equipped with a nanoelectrospray ion source
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