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Cells were washed three times with Krebs buffer (20 mM HEPES, 5 mM KH 2 PO 4 , 1 mM MgSO 4 , 1 mM CaCl 2 , 136 mM NaCl, 4.7 mM KCl, pH 7.4), then incubated with 500 g/ml 2-NBDG, a fluorescence-labeled deoxyglucose analog, for 20 min at room temperature
Rotation prevents cumulative tissue irritation and allows each site to fully recover before the next injection
What truly sets our approach apart is the comprehensive, personalized care that surrounds it
A standard starting dose of 0.25mg requires drawing to the 10-unit mark
US$ 28.54
US$ 21.06
US$ 28.78
US$ 20.22
