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Transfected HEK293T cells were treated in triplicate for 4 hours with a serial dilution of IGF-1 LR3 which had been pre-incubated in conditioned media for 7 days
This is followed by receptor autophosphorylation, and activation of receptor tyrosine kinases, which result in tyrosine phosphorylation of insulin receptor substrates (IRSs) including IRS1, IRS2, IRS3, IRS4, Gab1, and Shc [17]
furthermore, the number of studies that met the inclusion criteria was small, depression and anxiety themselves may affect fertility, and there is significant heterogeneity in the literature, especially in the measurement of fertility parameters
