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All measurements were normalized by the total protein levels and the results reported in moles of GSH per gram of protein
For the positive control group, silymarin was dissolved in 0.5% sodium carboxymethyl cellulose (Shanghai Shenguang Edible Chemicals Co., Ltd., Shanghai, China) as previously described ( To induce acute liver injury, CCl 4 was diluted to 1% in peanut oil and administered via oral gavage at a dose of 5 mL/kg, which is equivalent to 80 mg/kg CCl 4
The enhanced potency is due to the decreased binding of Long R3 IGF-1 to all known IGF binding proteins
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