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Deep anesthesia was induced using amobarbital sodium administered intraperitoneally at a dose of 150200 mg/kg, in accordance with the approved IACUC protocol and the AVMA Guidelines for the Euthanasia of Animals (AVMA, 2020) [31]

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Realistic skin improvements begin appearing around week 4-6 as the first collagen remodeling cycle completes and continues improving through week 12 and beyond