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Then, the FDA declared in late 2024 that tirzepatide was no longer in shortage, and it determined that semaglutide was no longer in shortage in early 2025

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Cells were then washed twice with M9, filtered through a 35-m cell strainer, and diluted 1:100 in enrichment media (brain heart infusion [BHI] media supplemented with 500 M l-carnitine)