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After blocking in 1% skim milk in Tw-PBS for 1 h at room temperature, the membrane was incubated with SMA (dilution 1:2000

Confirm whether the dose is written in mg or mcg before drawing Recalculate concentration if you ever change water volume Swirl, do not shake unless the product information explicitly says otherwise Write the reconstitution date the moment the vial is mixed, not later Treat any visual change as a reason to stop and verify Reconstituting different peptide types Most peptides reconstitute the same way mechanically, but the specifics diluent type, water volume, storage temperature vary by compound and source

Zhang 2012)

Cell Cycle 6, 26332639 (2007)