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The sections were incubated with a primary antibody specific for GPX4 or 4-HNE overnight at 4 C

Experimental protocol A total of 24 male Wistar Albino rats were randomly assigned into four groups ( n = 6 per group): Sham-operated control (SHAM), Bromelain only (BR), Ischemia-Reperfusion (IR), and Ischemia-Reperfusion with Bromelain treatment (IR + BR)

Keep it upright and out of direct light

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