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10.3390/ijms20143588 30 ContinM.LopaneG.MohamedS.Calandra-BuonauraG.CapellariS.MassisD.et al (2019)
Significantly, the yellow colouration observed in the optical microscopy images confirmed that binding of the substrates to the lipases was associated with bond cleavage, indicating that the enzymes remained functional in the solvent-free melt, and suggesting that the protein secondary structure and local configuration of the Ser-His-Asp triad at the active site were not significantly perturbed in the solvent-free biofluids
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