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Purified IVT-mRNA exhibits significantly lower immunogenicity, reduced induction of type I interferons (IFNs) and tumor necrosis factor (TNF-), and enhanced translational capacity of the encoded proteins ( Even with these purification and optimization techniques, the protein products of the IVT-mRNA retain some immunogenic properties, particularly the potential to elicit anti-drug antibodies (ADA) and pro-inflammatory cytokine responses, which may interfere with the desired therapeutic outcome
Oe Y, Omori T, Aimono E, Furukawa S, Kitakawa H, Tateno M, et al
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