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The cells were cultured in Eagles minimum essential medium (ThermoFisher Scientific) or DMEM F-12 (ThermoFisher Scientific) respectively, supplemented with 10% Fetal Bovine Serum (Gibco) and 1% penicillinstreptomycin (ThermoFisher Scientific), in a humidified incubator maintaining 5% CO 2 at 37 C

This lack of energy may make it difficult for individuals to engage in regular physical activity, potentially leading to weight gain

It also facilitates DNA synthesis and repair, stabilizes cellular membranes, and regulates appropriate immune responses (5-7)

Cyanocobalamin can be a prescription medication or an over-the-counter supplement, and is used to treat a variety of issues, including: Vitamin B12 Deficiency Pernicious Anemia, a condition where an autoimmune attack destroys the protein necessary for vitamin B12 absorption