To analyze the osmotic fragility of erythrocytes, different concentrations of sodium chloride solution were prepared (0.28%, 0.32%, 0.36%, 0.40%, 0.44%, and 0.48%)
One microgram of total RNA was used to perform reverse transcription with MmlV RT (Invitrogen) and oligo dT primers
An early study 25 addressed this question by genotyping 9 SNPs associated with serum UA in a series of PD and matched control subjects to obtain a polygenic risk score (PRS) for low UA
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