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It is noteworthy that compared with ERS agonist alone, the combination of ferroptosis inhibitor and ERS agonist can significantly reduce the incidence of ERS and cardiomyocyte injury, while ferroptosis agonist can aggravate cardiac dysfunction
Then, tissues were mechanically homogenized by passage through 70 m cell strainers, and the resulting homogenates were serially diluted in sterile PBS
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