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Sorted single-cell suspensions were resuspended at an estimated final concentration of 1000 cells/l and loaded on a Chromium GemCode Single Cell Instrument (10x Genomics) to generate single-cell gel beads-in-emulsion (GEM)
In addition, both human and mouse FSP1 cells exhibited similar sensitivity to viFSP1 with no significant difference in the calculated IC50 values
Intramuscular injections are generally inconvenient due to pain and require healthcare professionals for administration, thus increasing the economic burden on patients which ultimately leads to discontinuation of the treatment
When a lysate containing the GST-tagged protein is passed through a column containing immobilized glutathione, the GST moiety binds to the glutathione with high specificity and affinity, effectively capturing the fusion protein
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