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Following homogenization, 120 L of tissue homogenate was incubated with 0.2 mM mito-TEMPO-H in KrebsHenseleit KHB containing 100 M in 200 L of total volume for 1 h at 37 C

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423 The study began by inducing inflammation in mice through the injection of various Toll-like receptor (TLR) agonists and then assessed the inflammation by measuring plasma tumor necrosis factor-alpha (TNF-) levels