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Furthermore, ZIL aq.-based freezing media will provide some additional advantages: (1) the composition can be completely chemically defined, (2) the synthetic route is a completely chemical procedure, thus reducing the risk of unexpected infection, (3) the cost is low (~2 USD/100 mL 5% ZIL aq
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But if the question extends to energy homeostasis, feeding behavior, or the interplay between GH and metabolic state, that appetite signal becomes the most valuable variable in the study
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