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Quantification of 2HG enantiomers 2HG quantification in SCD erythroblasts was performed as we previously described 24 by liquid chromatographymass spectrometry (LC-MS/MS) after derivatization using N-(p-Toluenesulfonyl)-L-phenylalanyl Chloride (TSPC) on the LC-ESI-MS/MS system
"The clinical question is no longer whether microneedling-enhanced delivery works
Notably, the cross-sectional design and single-center recruitment limit generalizability, while seasonal variation in subtropical regions may confound baseline VD statusmethodological constraints requiring consideration in comparative analyses
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