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The cells were then washed three times with PBS containing 1% BSA (w/v) followed by 1 h incubation with 1:1,000 anti-mouse Alexa Fluor 488 conjugated secondary antibody (diluted with PBS containing 5% BSA, Invitrogen) at RT in the dark
Required documentation such as COA, specification sheet, allergen statement, and certification references
Severity of deficiency: if your levels were very low, it may take several doses to fully restore your tissues, or you may feel a dramatic early difference because your body was so depleted
Carnitine and ALC are structurally similar to choline and Ach
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