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Quantitative reverse transcription polymerase chain reaction (qRT-PCR) The total RNA of the hypothalamus and hindbrain was extracted with TRIzol Reagent, while the RNA of brown adipose tissue and liver was extracted by RNeasy Plus Mini Kit (#74136

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The conjugation of prostaglandin A1 and glutathione catalyzed by homogeneous [PMID: 1148268] Laboratory literature summary: The conjugation of prostaglandin A1 and glutathione catalyzed by homogeneous glutathione s-transferases from research subjects and rat liver

Adipose tissue is inherently pro-inflammatory, so losing weight naturally lowers inflammatory markers