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Dopamine transport, utilization, and storage may be involved in increased ROS formation followed by cellular dysfunction
Cytokine quantification Cytokine concentrations in culture supernatants and BALF samples were quantified using either LEGENDplex Mouse Th Panel or LEGENDplex Human Th2 Panel (BioLegend) per the manufacturers instructions and described previously (47, 71, 73, 75, 76)
They incorporated environmental and olfactory cues in CPP testing after two weeks of abstinence to assess cue-induced relapse vulnerability
The PCR products were gel-extracted, purified and cleaned (Macherey-Nagel, 740609) and 200 ng of each laccase mutant gene was transformed along with 100 ng of linearized vector into S
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