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In the present study, the detection of StAR and HSD31 in ram semen (seminal plasma + spermatozoa) by ELISA, and HSD32 protein expression by Western blot, suggests thatalthough not definitively proventhese molecules may either reach the sperm via a lumicrine route from the testis, or be synthesized by tissues of the genital tract or accessory sex glands during post-testicular transit
This high degree of stability ensures that longitudinal studies can be carried out without the risk of peptide denaturation or loss of potency over time, providing researchers with a reliable medium for high-fidelity data collection
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