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YasudaYNaganoTJimboNKiriuTSurayaRHazamaDet al
The 0.25 mg starting dose is intentionally sub-therapeutic for glycaemic control but serves as a tolerance-building phase
The ORF2 reading frame does not contain an AUG translation initiation codon and as such translation through ORF2 requires the failure of the ribosome to terminate at the end of ORF1 and instead shift to the +1 reading frame
(DMEM/F12, Gibco, Gaithersburg, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Gibco, Gaithersburg, USA) and 1% penicillin/streptomycin (Invitrogen, Life Technologies, USA) in a humidified incubator with 5% CO 2 at 37 C
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