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Mice were placed in a stereotaxic frame (Kopf Instruments, USA), and a rectangular craniotomy was performed over the medial prefrontal cortex (AP, 13 mm and ML, 01.5 mm from the bregma) using a dental drill (Microtorque II, Ram Products, USA)
Unlike full proteins, peptides are smaller and more easily absorbed, making them popular in medical treatments, fitness, and anti-aging therapies
Eur J Pharmacol 144:237239, 1987 Shwartz TW: Enteroinsular axis of pancreatic polypeptide
Drop back to the last tolerated dose and stay there for 6-8 weeks before attempting escalation again
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