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The charge state of every ionizable group -amino (pKa ~9), -carboxyl (pKa ~2), and each side chain is determined by the Henderson-Hasselbalch equation and the 2 rule
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Biophys J 107 , 18291840, (2014)
its the natural result of their bodys systems (like red blood cell production and nerve function) finally receiving the fuel they need to operate correctly
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