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After washing, the membranes were incubated with peroxidase-conjugated secondary antibodies (1:5000)

Chemical Structure and Amino Acid Sequence The published sequence carries several modifications relative to native GLP-1: 2-aminoisobutyric acid substitutions to resist enzymatic cleavage, an -methyl-leucine to alter conformation, a serinamide C-terminus, and a fatty-acid attachment that supports albumin binding for extended duration of action [3][4]