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8.1.3 Network biology approach While pathway analysis highlights the dysregulated processes, network-based approaches extend this by mapping the interaction between genes, proteins, and molecular regulators within complex cellular systems, offering a complete holistic view of system-level changes (287, 288)

NR4a1 knockdown ameliorates glucolipid metabolic disorders, mitochondrial dysfunction and pathological cardiac remodeling We further verified whether Sema affects Creb5/NR4a1 to improve TAC-induced pathological cardiac remodeling and metabolic dysfunction