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Anti-rabbit and Anti-mouse secondary antibodies conjugated to infrared fluorescent dyes (IRDye) were purchased from LI-COR Biosciences (Lincoln, NE, USA)

first discovered plasma intestinal flora-dependent small molecule metabolic spectrum by metabonomics and found that the plasma levels of TMAO and its precursors, choline and betaine, can predict the risk of human cardiovascular disease, thus identifying a novel relationship between intestinal flora and metabolic health (7)

Chemical Name: DSIP (Delta Sleep-Inducing Peptide) Molecular Formula: C35H48N10O15 Sequence: Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu (9-amino acid neuropeptide) Research Applications: Sleep cycle regulation studies Circadian rhythm modulation research Stress response and cortisol pathway analysis Pain perception and analgesic mechanism studies Hypothalamic-pituitary axis research Sleep architecture and REM cycle protocols Neuroendocrine system interaction studies Product Specifications: Form: Lyophilized powder Purity: High purity research grade Molecular Weight: 848.8 g/mol Storage: Store at -20C, protect from light and moisture Reconstitution: Use bacteriostatic water (not included) Stability: 24 months when stored properly, 30 days once reconstituted Research Background: DSIP is a naturally occurring neuropeptide originally isolated from the cerebral venous blood of rabbits in sleep-induced states

12 IV drips ( 3 months package)