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The other is glutamate incorporation into glutathione catalyzed by gamma-glutamylcysteine ligase (GCL), a rate limiting enzyme of glutathione synthesis (Sayin et al
The mixture was then passed through a 100-m cell strainer, pelleted at 500 g , resuspended in PBS, passed through a 40-m cell strainer and again pelleted at 500 g
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Chen YC, Chen YW, Hsu HS, Tseng LM, Huang PI, Lu KH, et al
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