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It helps eliminate bugs and pathogens
Procedure Preheat spectrophotometer/microplate reader for 30 minutes, adjust the wavelength to 412 nm, set zero with distilled Preheat Reagent II in 37 (mammal cell) or 25 (other species) water bath for 30 Blank tube determination: take micro glass cuvette, add 20 L of distilled water, 140 L of Reagent II, 40 L of Reagent III in turn, mix well, place for 2 minutes, and measure 412 nm absorbance AB
Put on a pair of nitrile gloves
As a proportion of Epi-cDC2s expresses langerin in the inner foreskin (but only a very low proportion expresses langerin in skin), coinciding with the high frequency of the LC2 subset in inner foreskin, it remains to be determined whether langerin-expressing Epi-cDC2s and LC2 represent the same or distinct LC subsets
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