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Leaf EL was estimated by using the method of 41 and the following formula: Measurement of non-enzymatic antioxidants Maize leaves (0.5 g) were homogenized in 3 mL ice-cold 5% meta-phosphoric acid containing 1 mM ethylenediaminetetraacetic acid (EDTA) using a mortar and pestle and centrifuged at 11,500 g for 12 min at 4 C

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Plotting the degree of reduction of the respective sensor variants against the redox potential of the ambient LA/DHLA buffer allowed to deduce E values of 237.7 2 mV for roGFP2-iL (Figure 4A) and 243.2 5 mV for GRX1-roGFP2-iL (Figure 4B)

Villagra-Blanco R, Silva LMR, Muoz-Caro T, Yang Z, Li J, Grtner U, Taubert A, Zhang X, Hermosilla C (2017) Bovine Polymorphonuclear Neutrophils cast Neutrophil extracellular traps against the abortive parasite Neospora caninum