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Both endothelial cells and aortic sections were treated with 0.5% (v/v) Triton X-100 for 15 min, followed by three washes with PBS, each time for 5 min, and then blocked with 5% (v/v) bovine serum albumin (BSA, Sigma-Aldrich, B2064) at room temperature for 1 h

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Confocal fluorescence microscopy Primary BMMs (3040% confluence) seeded onto a confocal dish were stimulated to osteoclast phenotype with 30 ng/mL M-CSF and 50 ng/mL RANKL and treated with 2.5 10 6 cells/mL SP for 30 min

This approach extends to our use of peptides and other therapeutic options