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Symptoms that are severe, or that last more than a few days and are not improving
Avoid freezing the reconstituted solution
For example, MD simulations show that cholesterol segregates neatly into the hydrocarbon region of SOPC (18:018:1) membranes up to a concentration of around 30 mol%, but above this threshold cholesterol is driven towards the center of the bilayer and its tilt angle relative to the bilayer normal increases (Figure 2B) [68]
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