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Pyridine sulfonyl derivatives of oestradiol (E2), 2-hydroxyoestradiol (2OH-E2), 4-hydroxyoestradiol (4OH-E2) but also of the internal standard d4-oestradiol (d4-E2) were profiled by LCtandem MS (LCMS/MS) using an instrument set-up containing an ultra-performance LC system (Aquity I-class, Waters) coupled to a triple quadrupole linear ion-trap mass spectrometer (QTRAP 6500+, Sciex)