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In Vitro Depletion of GSH and Generation of ROS by TNBS To examine the interaction of GSH with TNBS and the subsequent effect on GSH regulation, to an aqueous solution of GSH (1 mm) in Krebs-Henseleit buffer, an equimolar amount of TNBS was added and the level of GSH was examined by HPLC as described previously, which monitors reduced and oxidized GSH (Fariss and Reed, 1987)
miRNAs are small, mature, non-coding RNA molecules (about 22 nucleotides long) that can affect the processing of target mRNAs at the post-transcriptional level by translational inhibition or promoting mRNA degradation (281)
The mechanism of medicinal plants is broad, and includes regulation of intestinal microbiota composition and intestinal permeability, increased expression of both mRNA and claudin-1 protein, and anti-inflammatory effects (Chinese tea, hibiscus, liquorice, marshmallow, ginger root, peppermint, plantain, curcumin)
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