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Mutated GST sequences were subcloned into pET-15b, pET-24d, pGEX 4T-1, and pCMV-2B expression vectors, and the mutated constructs were confirmed by DNA sequencing
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Vitamin C helps "recycle" glutathione back into its active state, while glutathione helps restore vitamin C after it has neutralized a free radical, creating a powerful loop of antioxidant support
The pharmacokinetic parameters, including substrate affinity for P-glycoprotein (P-gp) and inhibitory potential against cytochrome P450 (CYP450) enzyme isoforms, were predicted using support-vector-machine-based binary classification models embedded within the SwissADME framework
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