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In stark contrast to the former two hydrolysis mechanisms, Hyaluronate lyase (EC 4.2.2.1): A polysaccharide lyase (PL-class) of microbial origin, which cleaves 1,4-glycosidic bonds in HA through an elimination mechanism, generating non-reducing end products with unsaturated carboncarbon bonds, as well as an unsaturated HA-disaccharide, namely 2-acetamido-2-deoxy-3-O-(-D-gluco-4-enepyranosyluronic acid)-D-glucose [17, 18]
