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A linear gradient from 2% (v/v) to 70% (v/v) mobile phase A was applied to retain less polar compounds, including NAC, NAM, and PGlu, while ensuring elution of strongly retained compounds within a reasonable time

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Research has explored its interactions with sleep architecture, neuroendocrine pathways, and stress-response mechanisms, with particular interest in its potential influence on growth hormone secretion and neuroprotective processes during sleep states