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Initial experiments were conducted with a wider potential window resulting in formation of both metallic copper and Cu(III)

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However, during an overdose these pathways become saturated and APAP is mostly metabolized by the Cytochrome P450 CYP2E1 and CYP3A4 isoenzymes into its reactive intermediate NAPQI (N-acetyl-p-benzoquinone imine) 2

Given that Glu is primarily neuroactive while Gln is storable and neuroinactive, the Gln/Glu ratio can reflect the efficiency of Glu uptake by astrocytes and its conversion to Gln, which is crucial for regulating excitatory neurotransmission [20]